Page 119 - Malaysian Journal of Health Promotion, Vol 4 (Supplementary 1) 2022

Malaysian Journal of Health Promotion, Vol 4 (Supplementary 1) 2022
14 MOH-AMM Scientific Meeting 2022 in conjunction with 23 NIH Scientific Conference Abstract Book
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ID 140 POTENTIAL MIRNA-MRNA INTERACTIONS RELATED TO NON-RESPONSIVENESS
TOWARDS IMATINIB IN CHRONIC MYELOID LEUKEMIA

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Aliza Mohd Yacob , Hamidah Akmal Hisham , Latifah Ibrahim , Ernie Zuraida Ali , Amir Firdaus Khorudin , Nor
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Asiah Muhamad and Yuslina Mat Yusoff 1
1 Institute for Medical Research, National Institutes of Health, Ministry of Health, Malaysia
2 School of Computing, Universiti Teknologi Malaysia
3 Sector for Evidence-based in Healthcare, National Institutes of Health, Malaysia

INTRODUCTION: Non-responsiveness towards imatinib among CML patients occurs around
30% and is shown as disease progression. A comprehensive approach to determine biological
networks of underlying factors is needed to improve treatment. Previous studies identified
three microRNAs as potential biomarkers for CML obtained using Miseq NGS; and two genes
that are likely exclusive for non-responsiveness towards imatinib obtained from Hiseq NGS.
Therefore, this study aims to identify potential miRNA-mRNA interactions related to non-
responsiveness towards imatinib in CML using bioinformatics prediction analysis.
METHODS: STarMirDB, a database-based prediction tool, was used to predict interactions
between three validated miRNAs and two genes obtained from previous studies. Interactions
in six different regions were looked at. These were seed and seedless regions at 3’UTR, CDS
and 5’UTR.
RESULTS: There were interactions predicted between each of hsa-miR-181a-5p, hsa-miR-26q-
5p, and hsa-miR-182-5p with PPIP5K2 in 3’UTR-seedless, and CDS-seed and -seedless regions.
Interaction between hsa-miR-181a-5p and PPIP5K2 was predicted in the 3’UTR-seed
region but not with the other two miRNAs. No interactions were predicted in 5’UTR-seed and
-seedless regions. There were interactions predicted between each of hsa-miR-181a-5p, hsa-
miR-26q-5p, and hsa-miR-182-5p with POLE3 all in seedless regions that were 3’UTR-seedless,
CDS-seedless, and 5’UTR-seedless. Interaction between hsa-miR-26a-5p and POLE3 was
predicted in the 3’UTR-seed region but not with the other two miRNAs. No interactions were
predicted in CDS-seed and 5’UTR-seed regions.
DISCUSSION/CONCLUSION: The unique interactions predicted for PPIP5K2 and POLE3 could
be related to non-responsiveness towards imatinib in these CML patients. Further analysis is
required to describe the interaction networks in detail.

ID 141 STRESS AMONG COVID-19 VACCINATION RECIPIENTS IN MALAYSIA

Zulkarnain Ramli
Centre of Communicable Diseases Research, IKU, National Institutes of Health

INTRODUCTION: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV2), which
causes the coronavirus disease 2019 (COVID-19), has infected and taken the lives of millions
of people around the world.
Social isolation and financial instability, together with a fear of contracting COVID-19 and
uncertainty of the future, pose substantial psychological stressors for the general population
it is likely that the pandemic induces a considerable degree of fear, worry and concern in the
general population.
This study aims to obtain descriptive information about stress using Perceive Stress Scale from
the vaccination Suveillance Program in Post Vaccination COVID-19 Imunity ang Disease
Surveillance In Malaysia (IMSURE).

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