Page 120 - Malaysian Journal of Health Promotion, Vol 4 (Supplementary 1) 2022

Malaysian Journal of Health Promotion, Vol 4 (Supplementary 1) 2022
rd
14 MOH-AMM Scientific Meeting 2022 in conjunction with 23 NIH Scientific Conference Abstract Book
th
METHODS: This study was all the respondents in IMSURE were asking using Perceive Stress
Scale (PSS). The Perceive Stress Scale is a classic stress assessment instrument. The Data than
analyzed using SPSS Ver 26.
RESULTS: There were 2504 respondent had been record from Julai 2021. There were 1271
(50.8%) male and 1233 (49.2% female. Ethnicity, there were 1598 (53.8%) Malays, 295
(11.8%) Chinese, 141 (5.6%) Indian, 65 (2.6%) Bumiputera Sabah, 39 (1.6%) Bumiputera
Sarawak and 366 (14.6%) Others. From the data, we get the Perceive Stress Score (PSS) levels
were low level, 237 (9.5%), moderate level, 1663 (66.4%) while the high level, 604 (24.1%).
A one way ANOVA revealed that there was a statistically significant difference in mean
Perceive Stress Score (PSS) between sex group (F(1, 2502)=[16.561], p=<0.001, Ethnicity
Group (F(5, 2498)=[7.740], p = < 0.001 and between age group (F(6, 2497)=[22.173], p = <
0.001.
DISCUSSION/CONCLUSION: There is a difference in Mean Perceive Stress Score (PSS)
according to statistics among the sex, age and gender group from the vaccine receiver during
IMSURE.

ID 142 AN EVALUATION OF THE MUTAGENICITYOFDIOSCOREA HISPIDA WATER EXTRACT

Norizah Awang, Elda Nurafnie Ibnu Rasid, Maizatul Hasyima Omar & Hussin Muhammad
Herbal Medicine Research Centre, Institute for Medical Research, National Institutes of Health, Ministry of
Health, Malaysia

INTRODUCTION: Dioscorea hispida or locally known as ‘Ubi gadung’ is a wild yam that is
consumed by local people. However, consumption of ‘Ubi gadung’ can cause accidental food
poisoning due to the presence of an alkaloid identified as dioscorine. Therefore, this study
was conducted to evaluate the mutagenicity effect of Dioscorea hispida and the content of
dioscorine in Dioscorea hispida extract.
METHODS: Dioscorea hispida was extracted with water and analyse using mass Spectrometry
to establish a chemical profile. The mutagenicity effects of the extract were determined by in
vitro bacterial reverse mutation assay (Ames test) using mutant strains of S.
Typhimurium strains TA98, TA100, TA1535, and TA1537, and Escherichia coli strain WP2uvrA.
RESULTS: The test results that produced revertant colonies were consistent and within the
range of the negative control. All the bacteria strains treated with and without metabolic
activation showed no increase of revertant colonies with the increase in the concentration of
Dioscorea hispida water extract up to 5000 µg/plate. Mass spectrometry analysis indicated
that no dioscorine present in the Dioscorea hispida water extract except for steroidal saponin
(dioscine).
DISCUSSION/CONCLUSION: Under the current test condition, water extract of Dioscorea
hispida without the presence of dioscorine has no reverse mutagenic potential. However,
more genotoxicity testing (micronucleus assay or comet assay) is necessary to be conducted
to establish the genotoxicity profile of Dioscorea hispida. The preparation of extract is
important because it contributes to the differences in the chemical profile that determines
the effect and efficacy of the extract.

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