Page 32 - IMR ANNUAL REPORT 2021: INSTITUTE FOR MEDICAL RESEARCH

IMR ANNUAL REPORT 2021 19

Chimerism Study

Chimerism analysis is used to monitor the success of engraftment or to
detect early graft failure, rejection and relapse after haematopoietic stem
cell transplant. This test evaluates the mixture of donor and recipient DNA
in the recipient’s blood or bone marrow using real-time quantitative PCR for
post-transplant monitoring. CENTRE (CaRC) CANCER RESEARCH

Haemato-oncology Laboratory Tests in 2021

2021
Type of Test Test Performed
Samples Tests
Leukaemia Translocation Leukaemia Fusion Genes 634 27,262
Study BCR-ABL 162 2,106

PML-RARA 26 286
FLT3-ITD 328 1,968
FLT3-D835 328 1,968
AML Mutation Study NPM1 227 2,724

CEBPA 136 3,128
c-KIT 44 264

BCR-ABL1 Kinase Domain Mutation Analysis 249 5,229
Chimerism Study 467 8380

Molecular Genetic Haematology Laboratory Tests

Molecular Thalassemia

Molecular thalassaemia tests offered for the diagnosis of thalassaemia are
the β amplification refractory mutation system (ARMS), β GAP, and ARMS
variant for haemoglobin E (HbE) thalassaemia. The laboratory also offers
additional panels for thalassaemia detection including:

• α ARMS
• α GAP
• α and β Multiplex Ligation-dependent Probe Amplification (MLPA)
• α-, β- and δ-sequencing
• A few modifiers i.e., XMN-1 polymorphism and triplication alpha