Page 126 - Malaysian Journal of Health Promotion, Vol 4 (Supplementary 1) 2022

Malaysian Journal of Health Promotion, Vol 4 (Supplementary 1) 2022
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14 MOH-AMM Scientific Meeting 2022 in conjunction with 23 NIH Scientific Conference Abstract Book
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ID 152 EUCHEUMA DENTICULATUM AS A FUNCTIONAL INGREDIENT TO PREVENT DIET-
INDUCED METABOLIC SYNDROMES IN RATS

Vimala Balasubramaniam, Siti Khadijah Abdul Khalid, Shirley Vino, Nurul Aznyda Norizan, Nazline Miasin Kehid,
Azizan Ali
Nutrition, Metabolism & Cardiovascular Research Centre, Institute for Medical Research, National Institutes of
Health, Ministry of Health

INTRODUCTION: Eucheuma denticulatum (ED) is a local red edible seaweed shown to have
various health-promoting effects and previous findings manifested its potential to prevent
signs of metabolic syndrome. The aim of this study is to substantiate the effectiveness of E.
denticulatum powder as a functional ingredient in a health drink using an in vivo model.
METHODS: To yield good quality dried powder, the seaweed was cleaned and washed
thoroughly to remove all the impurities, microbial contamination as well as heavy metals.
Following that, the seaweed was ground to a fine powder and was used a functional
ingredient in a health drink formulation. The nutritional composition of seaweed powder and
health drink was evaluated using standard AOAC methods. Eight to nine-week-old male
Sprague Dawley rats were randomly divided into five groups and fed for 8 weeks on a normal
chow diet, high‐fat diet (HFD), alone or supplemented with a 20% dried and milled ED as well
as health drink blended into the HFD diet while orlistat (50 mg/kg b.w.), a commercial anti-
obesity drug was administered via oral gavage daily as a positive control.
RESULTS: ED and health drink‐treated rats showed reduced body weight and adiposity,
improved liver and pancreas structure, improved liver enzyme markers and other metabolic
parameters.
DISCUSSION/CONCLUSION: The studies yielded significant information on the health benefits
of ED supplemented health drink to reduce metabolic syndrome biomarkers in in vivo model.

ID 153 DEVELOPMENT OF LYOPHILIZED HUMAN MESENCHYMAL STEM CELLS
DERIVEDEXOSOMES (hMSC-EVs) FOR THERAPEUTIC APPLICATIONS

Moon Nian Lim, Benson Koh, Pei Shan Poon, Noor Atiqah Fakharuzi, Rohaina Abd Razak, Gowrisankari, Shaik
Ahmad Kamal, Ezalia Esa, Jhi Biau Foo, Chee Wun How, Qi Hao Looi
Institute for Medical Research (IMR), National Institutes of Health (NIH), Ministry of Health Malaysia

INTRODUCTION: Extracellular vehicles (EVs) are small extracellular vesicles with phosphate
lipid bilayer, typically size ranging from 30-120 nm. EVs derived from human mesenchymal
stem cells (hMSCs) are one of the paracrine factors responsible for the functions of MSC such
as anti-inflammatory effect, improve angiogenesis and promote regeneration of tissue. Hence,
this study aims to develop a lyophilization method for the up-scale production and long-term
storage of EVs for therapeutic applications.
METHODS: Four units of human umbilical cord were collected with informed consent.
Harvested MSCs from cord tissues were cultured-expanded and EVs were isolated and
purified from the supernatant of the culture by polyethylene glycol-based (PEG) enrichment
method. The EVs were then lyophilized and subjected to characterization methods including
bicinchoninic acid (BCA) protein assay, nanoparticle tracking analysis (NTA),
chemiluminescent and fluorescent western blotting, and liquid chromatography-mass
spectrometry (LCMS) profiling.
RESULTS: NTA analysis showed that the isolated EVs remained at 43 – 46 nm particle size. All
exosomes’ samples were positive for CD9, CD63, and CD81 surface markers. Although a

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