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Scientific Data Contribution (NPHL)

NPHL is equipped with a high containment laboratory - Biosafety Level 3 (BSL3)
Laboratory, Virus Isolation laboratory, Electron Microscope Suites, Molecular Laboratory,
genomic sequencer and trained laboratory personnel. This allows NPHL to enhance its
approaches handling COVID-19.

The scientific data contribution mainly comes from IMR and I will share the NPHL scientific
contribution. NPHL is the first laboratory that identified and confirmed the presence of
SARS-CoV-2 virus in 3 out of 8 contact samples received from the first cluster (on the 24th
of January 2020). The positive samples identified were then cultured in Vero E6, MDCK,
MK2 and A549 cell lines which showed presence of Cytopathic Effect (CPE) in the Vero E6
cell line. The result was reconfirmed by Real Time Polymerase Chain Reaction (PCR).

Diagram 3 - Transmission Electron Microscope (FEI, Tecnai spirit G2 120kv) showed visible
coronavirus with crown.