Page 9 - ANIMAL MICROLOGY PRACTICAL EXERCISES IN ZOOLOGICAL MICRO-TECHNIQUE FOURTH REVISED EDITION BY MICHAEL F. GUYER

Contents xv

CHAPTER XVI. BACTEL 130
Bacterial Examination, 130; Cover-Glass Preparations from Fluid
Media, 130; Staining and Mounting, 131; Bacteria in Tissues, 131;
Methylen Blue Stain for Bacteria in Tissues, 132; Gram's Method
for Bacteria in Tissues, 132; Hanging-Drop Preparations, 132;
Memoranda, 133--36.
CHAPTER XVII. SOME EMBRYOLOGICAL METHODS: SECTIONS AND
WHOLE MOUNTS OF FROG AND CHICK; OTHER FORMS 137
The Frog, 137; Section Method, 137; Whole Mounts, 138; Mem-
oranda on Amphibian Material, 139-41; The Chick, 141; General
Memoranda on Embryological Methods and Materials, including
In Vitro Cultures, 143-57.
CHAPTER XVIII. SOME CYTOLOGICAL METHODS . 158
General Remarks, 158; Mitosis, 158; Testis of Crayfish, Sections,
159; Smears, 160; Blastodisk of Whitefish, 160; Testis of Necturus,
160; Somatic Cells of Ambystoma, 161; Living Cells, 161; Mito-
chondria, 162; Golgi Apparatus, 165; Staining of Living or Fresh
Tissues, 167; Tests for Certain Cellular Structures, 167; Special
Methods, 170; Allen's B-15 and B-20 Methods, 170; Hane's Cold
Method, 171; Photographing Cellular Structures, 172; Accessory
Chromosomes, 173; Aceto-Carmine Preparations, 173; Protoplas-
mie Currents, 174; Celloidin instead of Paraffin, 174; Urea in Fixing
Fluids, 174; Drop-Method of Changing Fluids, 174; Dehydration
by Dialysis, 175; Tissues of Young Adults Desirable, 175; Dissec-
tion of Living Cells, 175; Estimation of Carbon Dioxide, 176; Eu-
paral, 176; Memoranda, 173-78.
CHAPTER XIX. RECONSTRUCTION OF OBJECTS FROM SECTIONS 179
Reconstruction in Wax, 179; Geometrieal Reconstruction, 181;
Blotting Paper Method, 181; Rolling Drawings into the Wax, 181;
Photography in Reconstruction Work, 182; Cutting Out Wax
Plates, 182; Memoranda, 181, 182.

CHAPTER XX. DRAWING 183
Materials for Class Work, 183; Methods of Representation, 184;
Outline, 184; Depth, 184; Ink Drawings, 184; Pencil Drawings, 185;
Wash Drawings, 186; Size and Arrangement of Drawings, 187;
Labeling, 187; Modes of Representation for Special Courses, 188;
Embryology, 188; Histology, 189; Cytology, 189; Drawings for
Publication, 190; Materials for Manuscript Drawings, 190; Camera
Lueida, 191; Reduction, 191; Line Process, 191; Half-tone, 192;
Wash and Combination Drawings for Reproduction, 193; Lithog-
raphy, 194; Arrangement for Reductions, 194; Lettering, 194.