Page 7 - ANIMAL MICROLOGY PRACTICAL EXERCISES IN ZOOLOGICAL MICRO-TECHNIQUE FOURTH REVISED EDITION BY MICHAEL F. GUYER

CONTENTS
AO
INTRODUCTORY
Apparatus and Supplies Required, 1; General Rules, 5.

CHAPTER I. PREPARATION OF REAGENTS l
Practical Exercises, 7; Memoranda, 11-14.
CHAPTER II. GENERAL STATEMENT OF METHODS 15
Killing, Fixing, and Hardening, 15; Washing, 17; Dehydrating, 18;
Preserving, 19; Staining, 19; Clearing, 22; Mounting, 23; Imbed-
ding, 23; Affixing Sections, 24; Decolorizing, 25; Bleaching, 25; Cor-
rosion, 25; Decaleification and Desilicidation, 25; Injection Meth-
ods, 26; Isolation of Histologieal Elements, 26; Normal or Indif-
ferent Fluids for Examining Fresh Tissues, 27; General Scheme for
Mounting Whole Objects (In Toto Preparations) or Sections, 27.

CHAPTER IHI. KILLING AND FIXING 28
Cautions, 28; Fixing with Zenker's Fluid, 29; Fixing with Bouin's
Fluid, 30; Formalin as a Fixing Reagent, 30; Memoranda, 31-34.

CHAPTER IV. SIMPLE SECTION METHODS • 35
Free-hand Section Cutting, 35; Memoranda, 36, 37.
CHAPTER V, 'THE PARAFFIN METHOD: INFILTRATION AND SECTIONING. 38
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The Method, 38; Memoranda, 44 ; Difficulties Likely To Be En-
countered in Sectioning in Paraffin, and the Probable Remedy, 48.
CHAPTER VI. 'THE PARAFFIN METHOD: STAINING AND MOUNTING 52
Staining with Hematoxylin, 52; Double Staining in Hematoxylin
and Eosin, 54; Double Staining in Cochineal and Lyons Blue, 54;
Staining with Heidenhain's Iron-Hematoxylin, 54; Iron-Hema-
toxylin with Other Stains, 56; Staining in Bulk before Sectioning,
56; Paraffin Method for Delicate Objects, 57; Euparal as a Mount-
ing- and Preservation-Medium, 58; Memoranda, 58-63,
CHAPTER VIL. THE DIOXIN METHOD . 64
Why Valuable, 64; The Method, 64; With the Freezing Method,
67; Memoranda, 65-67.
CHAPTER VIII. THE CELLOIDIN METHOD . 0
The Method, 68; Staining Celloidin Sections in Hematoxylin and
Eosin, 71; Memoranda, 7I-76.
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