Page 98 - NATIONAL INSTITUTES OF HEALTH (NIH) GUIDELINES FOR CONDUCTING RESEARCH IN MINISTRY OF HEALTH (MOH) INSTITUTIONS & FACILITIES 3RD EDITION 2021

death as death as end point; production of radiation
sickness, certain injections, and stress and shock
research that would result in pain approaching the pain
tolerance threshold i.e. the point at which intense
emotional reactions occur. Category D experiments
present an explicit responsible on the investigator to
explore alternative designs to ensure that animal distress
is minimized or eliminated.

Category E
Procedures that Use of muscle relaxants or paralytic drugs such as
involve inflicting severe succinyl choline or other curariform drugs used alone for
pain near, at, or above surgical restraint without the use of anesthetics; severe
the pain tolerance burn or trauma infliction on unanesthetized animals;
threshold of attempts to induce psychotic-like behavior; killing by use
unanesthetized, of microwave ovens designed for domestic kitchens or by
conscious animals. strychnine; inescapably severe stress or terminal stress.
Category E experiments are considered highly
questionable or unacceptable irrespective of the
significance of anticipated results. Many of these
procedures are specifically prohibited in national policies
and therefore may result in withdrawal of federal funds
and/or institutional USDA registration.

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82 NIH Guidelines for Conducting Research in MOH, 2021