Page 63 - INSTITUTE FOR MEDICAL RESEARCH ANNUAL REPORT 2020

IMR Annual Report 2020 47

Diagnostic Tests Performed at Bacteriology Unit in 2020

NO. DIAGNOSTIC TEST TEST

1 Melioidosis serology (ELISA IgM detection) 7552
2 Leptospira total antibody (MAT) 2007
3 MTB real-time PCR and MOTT detection 2809

4 Leptospira PCR 908
5 Serotyping Streptococcus pneumoniae 1082
6 Fungal identification (biochemical and PCR) and antifungal susceptibility testing 1785
7 CRE detection PCR 1694

8 MIC Colistin and Colistin microbroth dilution 1286 Research Centre (IDRC) Infectious Diseases
9 Bordetella pertussis PCR 724
10 PCR 16s RNA for bacterial ID and B. pseudomallei PCR 341
11 ID of aerobes 291
12 Rickettsia IIP test (IgM and IgG) 58
13 Brucella species antibody detection test (IgM, IgG) 193

Total number of tests 20, 730

Services provided by Medical Entomology Unit in 2020

Malaria and Filaria

Every year, mosquito samples from malaria/filaria endemic areas are sent to IDRC from the State
Health Department. A total of 46 Molecular PCR tests were conducted for detection of malaria
parasite and filarial worm in mosquitoes in 2020. No filaria outbreak was reported. There was a
reduction of number of mosquito samples received in 2020, during the pandemic, as compared to
a total of 2008 samples received in the previous year.

Forensic Entomology

Diagnostic service for forensic entomology in estimating the minimum post-mortem interval
(mPMI) for crime investigations and also identification of insects is also carried out. The procedures
consist of mounting for larvae, colonisation from larvae until adult and molecular detection for
species confirmation. In 2020, the unit received 92 cases of forensic entomology and two cases of
myiasis. In comparison, the unit received 127 forensic entomology cases in 2019.