Page 2 - Flow Cytometry Protocols Fourth Edition

Contents

Pref ace . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
1 Flow Cytometry: The Glass Is Half Full.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Howard M. Shapiro
2 High- Dimensional Modeling for Cytometry: Building Rock
Solid Models Using GemStone™ and Verity Cen-se'TM
High-Definition t-SNE Mapping . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
C. Bruce Bagwell
3 Mass Cytometry Assays for Antigen-Specific T Cells Using CyTOF . . . . . . . . . . . 37
Dongxia Lin and Holden T. Maecker
4 RNA Flow Cytometry Using the Branched DNA Technique................. 49
Kah Teong Soh and Paul K. Wallace
5 Analysis of Individual Extracellular Vesicles by Flow Cytometry. . . . . . . . . . . . . . . 79
John P. Nolan and Erika Dungan
6 Quantitative Fluorescence Measurements with Multicolor
Flow Cytometry..... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 3
Lili Wang, Adolfas K. Gaigalas, and James Wood
7 High Throughput Flow Cytometry for Cell Surface Profiling... . . . . . . . . . . . . . 111
Joshua Paterson and Laurie E. Ailles
8 Multiparameter Conventional Flow Cytometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139
Katherine M. McKinnon
9 Multiparameter Intracellular Cytokine Staining . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
Patricia Lovelace and Holden T. Maecker
10 Multiparametric Analysis of Apoptosis by Flow Cytometry................... 167
William G. Telford
11 Multiparameter Cell Cycle Analysis ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203
James W. Jacobberger, R. Michael Sramkoski,
Tammy Stefan, and Philip G. Woost
12 Monitoring Cell Proliferation by Dye Dilution: Considerations
for Probe Selection...................................................... 249
Joseph D. Tario Jr., Alexis N. Conway, Katharine A. Muirhead,
and Paul K. Wallace
13 Immunophenotypic Identification of Early Myeloerythroid
Development........................................................... 301
Cornelis J.H. Pronk and David Bryder
14 Flow Cytometry Assays in Primary Immunodeficiency Diseases............... 321
Maurice R.G. OGorman

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